Skip to main content

Advertisement

Table 1 Primary antibodies.

From: Discrete nuclear structures in actively growing neuroblastoma cells are revealed by antibodies raised against phosphorylated neurofilament proteins

Antibody Species/Isotype Dilution for Fluorescence Dilution for Western Analysis Supplier
Anti-BrdU mAB Mouse IgG 1:400 in PBS   Molecular Probes, Eugene, OR (#A-21300)
Anti-GFAP pAB Rabbit IgG 1:86 in PBS 1:400 in PBS Sigma Chem. Co., St. Louis, MO (#G-9269)
Anti-lamin A/C mAB Mouse IgG   1:500 Chemicon International, Temecula CA (MAB3538)
Anti-lamin B mAB Mouse IgG   1:500 Chemicon International, Temecula CA (MAB3536)
Anti-neurofilament, RT-97 Mouse IgG Undiluted culture supernatant Undiluted culture supernatant Developmental Studies Hybridoma Bank at the University of Iowa, Iowa City, IA
Anti-nonphospho-neurofilament, SMI-32 mAB Mouse IgG1 1:1000 in PBS 1:1000 in PBS Sternberger Monoclonals, Inc. Lutherville, MD
Anti-phospho-neurofilament, SMI-31 mAB Mouse IgG1 1:1000 in PBS 1:2000 in PBS Sternberger Monoclonals, Inc., Lutherville, MD