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Table 1 Primary antibodies.

From: Discrete nuclear structures in actively growing neuroblastoma cells are revealed by antibodies raised against phosphorylated neurofilament proteins

Antibody

Species/Isotype

Dilution for Fluorescence

Dilution for Western Analysis

Supplier

Anti-BrdU mAB

Mouse IgG

1:400 in PBS

 

Molecular Probes, Eugene, OR (#A-21300)

Anti-GFAP pAB

Rabbit IgG

1:86 in PBS

1:400 in PBS

Sigma Chem. Co., St. Louis, MO (#G-9269)

Anti-lamin A/C mAB

Mouse IgG

 

1:500

Chemicon International, Temecula CA (MAB3538)

Anti-lamin B mAB

Mouse IgG

 

1:500

Chemicon International, Temecula CA (MAB3536)

Anti-neurofilament, RT-97

Mouse IgG

Undiluted culture supernatant

Undiluted culture supernatant

Developmental Studies Hybridoma Bank at the University of Iowa, Iowa City, IA

Anti-nonphospho-neurofilament, SMI-32 mAB

Mouse IgG1

1:1000 in PBS

1:1000 in PBS

Sternberger Monoclonals, Inc. Lutherville, MD

Anti-phospho-neurofilament, SMI-31 mAB

Mouse IgG1

1:1000 in PBS

1:2000 in PBS

Sternberger Monoclonals, Inc., Lutherville, MD