Dietary protein restriction causes modification in aluminum-induced alteration in glutamate and GABA system of rat brain

Table 3 Regional GAD activity (μmoles of GABA produced / hr / mg wet tissue) of brain.

Brain regions	Groups of animals				Statistical calculation (F)
	Normal protein		Low protein		ANOVA Single factor	ANOVA Two factors (with replication)
	Control	Aluminum exposed	Control	Aluminum exposed
Cerebrum	30.91 ± 2.18	57.67 ± 1.62 *	41.65 ± 2.60	31.47 ± 1.55 *	37.93 ^#	Protein : 14.48 ^# Aluminum : 13.68 ^# Interaction : 82.63 ^#
Thalamic area	40.14 ± 1.16	47.15 ± 2.57	44.92 ± 2.78	42.80 ± 1.33	2.05	Protein : 0.01 Aluminum : 1.37 Interaction : 4.77 ^#
Midbrain-hippocampal region	38.48 ± 2.35	52.53 ± 0.77 *	43.48 ± 1.80	38.82 ± 2.81	9.93 ^#	Protein : 4.40 ^# Aluminum : 5.11 ^# Interaction : 20.27 ^#
Cerebellum	25.81 ± 0.87	31.47 ± 2.02	34.04 ± 1.69	24.67 ± 2.21 *	6.24 ^#	Protein : 0.24 Aluminum : 1.28 Interaction : 17.22 ^#

Data are means of six observations ± SEM. * indicates significant difference (p < 0.05) in comparison to respective control. # indicates calculated F is higher than the critical F (α = 0.05).

ISSN: 1471-2202