Neurofilament H and tyrosine hydroxylase expression in cultures derived from sfEB/MedII. (A) Neurofilament H expression in neurons merged with DAPI staining of all nuclei. (B) Tyrosine hydroxylase expression in a small network of neurons in plated sfEB(MedII) derived from BG01 cells. The background fluorescence is caused by out of focus TH+ neurons. Anti-TH immunostaining was not performed on differentiations of BG02 cells. Images and scale bars: (A) 40×, 25 μm. (B) 60×, 25 μm.