Nestin and MAP-2 expression in cultures derived from plated sfEB/MedII. (A-E) and (F-I) represent images of two different fields. (A,F) Phase contrast image of rosettes, neurons and surrounding cells. (B,G) DAPI stained nuclei. (C) nestin expression in rosettes and surrounding cells. (D,H) Map-2 expression showing individual neurons and radial expression in rosettes (arrows). (I) Overlay of (G,H). (E) Overlay of (B-D). (J) An independent field showing Map-2 expression in a network of associated neurons. Images and scale bars: (A-J) 40×, 25 μm.