Double immunofluorescence for MIF and GFAP in the lumbosacral spinal cord. Each panel is a composite of two different photographs, arranged to overlap each other using Adobe Photoshop. A) In the dorsal funiculi, GFAP immunostaining (red) was very prominent. MIF immunostained cells (green) were seen associated with GFAP fibers. Arrow points to midline. In the dorsal horn (B & C), many of the MIF immunostained cells (green) were associated with GFAP immunofluorescence (red). However, some cells appeared to contain only MIF immunostaining (arrow). D. Several MIF immunostained cells are visible in the IML region. All of them also reveal GFAP staining. Calibration bar = 100 μm.