MIF immunohistochemistry in the lumbosacral spinal cord. A) Low power view of a section processed for MIF PAP. Many intensely labeled cells are observed in the white mater, the dorsal horn and in the IML region. Diffuse staining was observed in the dorsal horn. Moderately stained cells can be seen throughout the intermediate grey and in the area around the central canal. B) Higher magnification of the area of the dorsal horn showing labeled profiles. In addition, the dorsal root entry zone (marked with an asterisk) also appears labeled with MIF. Diffuse staining over the dorsal horn is marked by the arrows. C) Weak to moderately stained cells in the around the central canal. D) MIF stained cells were also observed in the intermediolateral cell region (IML), a region that contains sacral parasympathetic preganglionic neurons and interneurons. Calibration bar, A = 400 um; B-D = 100 um.