MIF immunohistochemistry in the peripheral ganglia of the rat. Neurons in the MPG showed weak to moderate MIF immunostaining (Fig 2A). Some of these neurons also co-contained TH (Fig. 2B). Double arrows illustrate neurons that contained MIF and also contained TH, marked in panel 2B with single arrows. However, some of the neurons that were stained with MIF (Single arrow) were not TH-positive (* in 2B). Therefore, both sympathetic and parasympathetic neurons in the MPG contained MIF. MIF was detected in neurons in the IMG (Fig 2C) and in neurons in the lumbar sympathetic chain (Fig 2E). Arrowheads indicate neurons that were identified as containing MIF and TH (Fig 2D,2F) in the IMG and the sympathetic chain. SIF cells in the IMG (large arrowhead) also contained MIF and TH (Fig. 2C,2D). Many of the neurons in the L6/S1 DRG stained for MIF. Weak to moderate immunostaining was observed in the cytoplasm of the neurons (Fig. 2G). Arrows illustrate some of the more intensely stained MIF containing neurons that also contained substance P (Figure 2H) In addition, the satellite cells surrounding the neurons were intensely stained for MIF. One such example is marked with double arrowheads in Fig. 2G, however, other examples are visible but not marked. Calibration bar = 100 μm.