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Figure 2 | BMC Neuroscience

Figure 2

From: ALS-associated mutant SOD1G93A causes mitochondrial vacuolation by expansion of the intermembrane space and by involvement of SOD1 aggregation and peroxisomes

Figure 2

SOD1 is concentrated at the boundary of vacuoles and in focal areas inside vacuoles, while cytochrome c oxidase subunit 1 (CO1) is located only in focal areas inside vacuoles. (A, B) Spinal cord sections from SODG93A mice were doubly stained for SOD1 (red) and CO1 (green). Vacuoles are photographed using a confocal microscope and viewed by 3-d reconstruction. The right panels show the superimposition of the red and green signals. The two right-most panels are viewed from a 30° angel. White circles are added to the top and bottom to aid visualization of 3-d structure. Arrows point to small vesicular structure inside vacuoles. Arrowheads point to SOD1 aggregates.(C, D) Spinal cord sections from SODG93A mice were doubly stained for SOD1 (small particles) and CO1 (large particles). D is a high magnification view of the mitochondrial matrix remnants pointed by the arrowhead in C. The arrow points to a vesicular structure inside the vacuole. The arrowhead points to the inner membrane remnants within this vacuole.

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