Neurons labeled from either the bladder or the prostate three days post infection Maps of sections from the lumbosacral spinal cord showing neurons labeled from either the bladder or the prostate three days post infection. Neurons labeled by virus that infected the bladder are red ("x" or "o") and those that are labeled by virus that infected the prostate are green ("x" or "o"). The "o" means that the neuron is also cholinergic (preganglionic). Blue neurons ("x") are cholinergic neurons that are not labeled by either virus. A: Section from the L6-S1 spinal cord. Cholinergic bladder neurons (parasympathetic preganglionic) are restricted to the intermediolateral area. Bladder neurons that are not cholinergic are found in the IML and DGC (interneurons) and in SDH. Cholinergic neurons that are not labeled by either virus (blue "x") are found in the ventral horn (somatic motor neurons), the intermediolateral area (preganglionic motor neurons that innervate other visceral organs) and around the central canal. Note that, in this particular section, there are no neurons labeled by virus that was injected into the prostate. B: Section from the L1-L2 spinal cord. Note that there are neurons labelled by virus that infected the bladder as well as the prostate. There are cholinergic (preganglionic, located in the intermediolateral and commissural areas) and non-cholinergic neurons labeled by virus from both organs. Bar: 1 mm.