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Table 2 Effects of the cryb mutation on BG-luc and tim-luc reporter activity in antennal specimens under light-dark cycles (LD 12:12), constant darkness (DD and constant temperature (HH)1,2

From: Advanced analysis of a cryptochrome mutation's effects on the robustness and phase of molecular cycles in isolated peripheral tissues of Drosophila

Genotype3 Na4 Number5 Rhythmic (statistical significance) Period (Mean ± SEM) Rhythmicity6 Index (Mean ± SEM) Rhythmicity Statistic (Mean ± SEM) Rhythm Amplitude (Mean ± SEM) Phase Activity Counts (MeanfSEM)
LD
BG-luc; cry + 36 33 (23) 24.2 ± 0.3 0.49 ± 0.03 3.0 ± 0.2 0.17 ± 0.01 2.8, 0.9 335 ± 33
BG-luc; cry b 41 16 (3) 25.8 ± 1.0 0.12 ± 0.06 0.8 ± 0.4 0.09 ± 0.02 8.2, 0.9 299 ± 65
tim-luc; cry + 20 16 (9) 25.0 ± 0.3 0.36 ± 0.05 2.3 ± 0.3 0.15 ± 0.02 2.1,0.9 309 ± 30
tim-luc; cry b 36 11 (1) 26.5 ± 1.0 0.23 ± 0.05 1.4 ± 0.3 0.09 ± 0.01 9.2, 1.0 477 ± 105
DD
BG-luc; cry + 38 33(4) 25.7 ± 0.4 0.20 ± 0.03 1.2 ± 0.2 0.12 ± 0.01 5.5, 1.0 404 ± 27
BG-luc; cry b 31 14(1) 26.8 ± 1.3 0.03 ± 0.04 0.2 ± 0.2 0.02 ± 0.00 10.5,0.3 350 ± 75
tim-luc; cry + 23 22(0) 26.4 ± 0.4 0.12 ± 0.03 0.7 ± 0.2 0.08 ± 0.00 5.4, 0.8 269 ± 40
tim-luc; cry b 37 8(0) 24.3 ± 2.2 -0.1 ± 0.09 -0.6 ± 0.5 0.04 ± 0.00 7.9, 0.5 625 ± 109
HH
BG-luc; cry + 112 94(5) 25.4 ± 0.3 0.12 ± 0.01 0.7 ± 0.1 0.08 ± 0.00 -0.1,0.6 487 ± 29
BG-luc; cry b 107 28(2) 25.4 ± 0.5 0.14 ± 0.03 0.9 ± 0.2 0.06 ± 0.00 0.9, 0.6 624 ± 98
tim-luc; cry + 112 74(0) 25.3 ± 0.3 0.14 ± 0.01 0.8 ± 0.1 0.09 ± 0.00 0.2, 0.7 902 ± 73
tim-luc; cry b 80 37(4) 25.8 ± 0.2 0.11 ± 0.02 0.7 ± 0.1 0.08 ± 0.00 -1.4,0.5 674 ± 90
  1. 1) The experimental conditions are described in Krishnan et al [23] and in Materials and Methods. 2) Ambient conditions are light-dark cycles with 12 hours of light and 12 hours of darkness (LD 12:12) or constant darkness at 25°C or constant temperature in which the specimens were first synchronized to temperature cycles in constant darkness consisting of 12 hours of 18°C followed by 12 hours of 27°C before they were evaluated under a constant high temperature of 27°C (HH). See Materials and Methods for further details. Also see Krishnan et al. [23]3) All headings in Table 2 are defined as in Table 1. 4) The analysis was applied to samples previously described in Krishnan et al [23]. The number of specimens analyzed (Na) in LD and DD is unchanged, while the number of specimens in HH has increased by 123 additional cry+ antennal pairs and 62 additional cryb specimens (see Materials and Methods for more details) 5) We asked whether cryb affects rhythmicity in antennal pairs for each ambient condition. Chi-squared tests showed significant effects of the mutation on rhythmicity with either BG-luc or tim-luc in all cases (p < .001). 6) The cryb mutation significantly reduced the RI value for each reporter in LD and DD (t-test, p < .001) but not HH (p > .05).