The 200 kDa band represents a dimer of VR1 subunits. Western blot of oocytes expressing wild-type VR1, Δ2–52, or wild-type VR1 + Δ2–52. Oocytes were prepared as described in Experimental Procedures. The band observed for VR1-express ing oocytes was at 200 kDa, the band observed for the Δ2–52-expressing oocytes was at 180 kDa, while oocytes injected with a 1:1 ratio of VR1 and Δ2–52 RNA yield three bands of 200 kDa, 180 kDa, and 190 kDa. This blot was probed with the FLAG antibody.