Small interfering RNA (siRNA) particles decreases AGRP mRNA. (a) Synthetic siRNA particle designed against AGRP mRNA. (b) Representative bands from the Northern blot 24 hours after injection shows decreased levels of AGRP mRNA in the group injected with AGRP-siRNA particle compared to the control GFPi-siRNA injections. (c) Quantification of the Northern blot results yields a statistically significant decrease in expression of 50% with the AGRP-siRNA particle. Data are expressed as a mean percentage ± SEM of GFP-siRNA levels (n = 16; p < 0.05).