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Table 2 Comparison of microarray, quantitative Northern slot blot and in situ hybridization data for genes validated in this study.

From: Replicate high-density rat genome oligonucleotide microarrays reveal hundreds of regulated genes in the dorsal root ganglion after peripheral nerve injury.

  

Array Data

Northern Data

In situ

Known

Descriptions

Accession Number

Naïve Mean intensity ± S.D.

Axotomy Mean intensity ± S.D.

Fold change

P value

 

Fold change

P value

 

Regulation

Regulation

GTP cyclohydrolase I

M58364

222 ± 12

1605 ± 194

7.2

0.0064

**

3.8

0.0000

***

↑

 

Endothelin-1

M64711

538 ± 170

716 ± 34

1.3

0.2076

 

1.7

0.0004

***

  

Cannabinoid CB1 receptor

X55812

576 ± 21

388 ± 49

-1.5

0.0118

*

-1.2

0.4221

   

Cholecystokinin-B receptor

M99418

588 ± 145

534 ± 56

-1.1

0.6011

 

2.7

0.0008

***

 

↑(31,32)

53 kD polypeptide

X02601

645 ± 194

723 ± 113

1.1

0.5875

 

2.4

0.0433

*

  

Small proline-rich protein 1a (EST195714)

AA891911

1063 ± 205

3856 ± 263

3.6

0.0002

***

3.3

0.0267

*

 

↑(8)

ET-B endothelin receptor

X57764

1228 ± 1015

1448 ± 1131

1.2

0.8145

 

1.2

0.1445

   

Guanine nucleotide-releasing protein (MSS4)

L10336

1301 ± 335

1286 ± 174

1.0

0.9478

 

-1.1

0.0562

   

Immediate-early serum-responsive JE (IES-JE)

X17053

1539 ± 473

3864 ± 818

2.5

0.0208

*

2.0

0.0089

**

↑

 

Peripheral-type benzodiazepine receptor

J05122

2664 ± 314

4148 ± 459

1.6

0.0132

*

2.1

0.0078

**

  

5HT-3 receptor

U59672

2750 ± 254

1413 ± 276

-1.9

0.0036

**

-2.6

0.0140

*

↓

 

Gadd45

L32591

3394 ± 195

14,021 ± 644

4.1

0.0005

***

5.9

0.0047

**

  

Pituitary adenylate cyclase activating peptide(PACAP)

X80290

3466 ± 434

12,182 ± 290

3.5

0.0000

***

4.6

0.0004

***

 

↑(6,55)

Phopholemman chloride channel (EST189142)

AA799645

3629 ± 327

8094 ± 283

2.2

0.0001

***

1.6

0.0062

**

  

VGF (nerve growth factor-inducible protein)

M74223

3791 ± 619

10,200 ± 748

2.7

0.0004

***

2.7

0.0050

**

↑

 

HNF-3/fork-head homolog-2 (HFH-2)

L13202

3961 ± 396

3640 ± 202

-1.1

0.2994

 

1.3

0.0986

   

GFRα1(RET ligand 1)

U97142

4132 ± 755

8443 ± 581

2.0

0.0019

**

2.4

0.0029

**

 

↑(45)

CLP36 (Elfin)

U23769

5124 ± 532

11,527 ± 666

2.2

0.0003

***

2.5

0.0019

**

  

Metallothionein-1 (EST211851)

AI102562

5628 ± 779

6715 ± 319

1.2

0.1230

 

1.6

0.0321

*

  

Calcium channel α-2 subunit (CCHL2A)

M86621

7267 ± 1792

25,005 ± 1485

3.4

0.0002

***

4.7

0.0125

*

↑

↑(65)

α-2-macroglobulin

M23566

8995 ± 1046

21,057 ± 3221

2.3

0.0157

*

2.6

0.0023

**

  

Lysozyme (EST196578)

AA892775

10,702 ± 3948

26,572 ± 2127

2.5

0.0082

**

2.9

0.0024

**

  

Guanine nucleotide-binding protein G-i, α subunit

M12672

10,728 ± 1325

10,781 ± 1099

1.0

0.9608

 

1.1

0.0217

*

  

SNAP-25A

AB003991

22,532 ± 3763

16,441 ± 1731

-1.4

0.0898

 

-1.4

0.0486

*

↓

 
  1. Key to abbreviations, * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed unpaired t-test). Down regulated genes are expressed as negative fold changes.