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Table 2 Comparison of microarray, quantitative Northern slot blot and in situ hybridization data for genes validated in this study.

From: Replicate high-density rat genome oligonucleotide microarrays reveal hundreds of regulated genes in the dorsal root ganglion after peripheral nerve injury.

   Array Data Northern Data In situ Known
Descriptions Accession Number Naïve Mean intensity ± S.D. Axotomy Mean intensity ± S.D. Fold change P value   Fold change P value   Regulation Regulation
GTP cyclohydrolase I M58364 222 ± 12 1605 ± 194 7.2 0.0064 ** 3.8 0.0000 ***  
Endothelin-1 M64711 538 ± 170 716 ± 34 1.3 0.2076   1.7 0.0004 ***   
Cannabinoid CB1 receptor X55812 576 ± 21 388 ± 49 -1.5 0.0118 * -1.2 0.4221    
Cholecystokinin-B receptor M99418 588 ± 145 534 ± 56 -1.1 0.6011   2.7 0.0008 ***   ↑(31,32)
53 kD polypeptide X02601 645 ± 194 723 ± 113 1.1 0.5875   2.4 0.0433 *   
Small proline-rich protein 1a (EST195714) AA891911 1063 ± 205 3856 ± 263 3.6 0.0002 *** 3.3 0.0267 *   ↑(8)
ET-B endothelin receptor X57764 1228 ± 1015 1448 ± 1131 1.2 0.8145   1.2 0.1445    
Guanine nucleotide-releasing protein (MSS4) L10336 1301 ± 335 1286 ± 174 1.0 0.9478   -1.1 0.0562    
Immediate-early serum-responsive JE (IES-JE) X17053 1539 ± 473 3864 ± 818 2.5 0.0208 * 2.0 0.0089 **  
Peripheral-type benzodiazepine receptor J05122 2664 ± 314 4148 ± 459 1.6 0.0132 * 2.1 0.0078 **   
5HT-3 receptor U59672 2750 ± 254 1413 ± 276 -1.9 0.0036 ** -2.6 0.0140 *  
Gadd45 L32591 3394 ± 195 14,021 ± 644 4.1 0.0005 *** 5.9 0.0047 **   
Pituitary adenylate cyclase activating peptide(PACAP) X80290 3466 ± 434 12,182 ± 290 3.5 0.0000 *** 4.6 0.0004 ***   ↑(6,55)
Phopholemman chloride channel (EST189142) AA799645 3629 ± 327 8094 ± 283 2.2 0.0001 *** 1.6 0.0062 **   
VGF (nerve growth factor-inducible protein) M74223 3791 ± 619 10,200 ± 748 2.7 0.0004 *** 2.7 0.0050 **  
HNF-3/fork-head homolog-2 (HFH-2) L13202 3961 ± 396 3640 ± 202 -1.1 0.2994   1.3 0.0986    
GFRα1(RET ligand 1) U97142 4132 ± 755 8443 ± 581 2.0 0.0019 ** 2.4 0.0029 **   ↑(45)
CLP36 (Elfin) U23769 5124 ± 532 11,527 ± 666 2.2 0.0003 *** 2.5 0.0019 **   
Metallothionein-1 (EST211851) AI102562 5628 ± 779 6715 ± 319 1.2 0.1230   1.6 0.0321 *   
Calcium channel α-2 subunit (CCHL2A) M86621 7267 ± 1792 25,005 ± 1485 3.4 0.0002 *** 4.7 0.0125 * ↑(65)
α-2-macroglobulin M23566 8995 ± 1046 21,057 ± 3221 2.3 0.0157 * 2.6 0.0023 **   
Lysozyme (EST196578) AA892775 10,702 ± 3948 26,572 ± 2127 2.5 0.0082 ** 2.9 0.0024 **   
Guanine nucleotide-binding protein G-i, α subunit M12672 10,728 ± 1325 10,781 ± 1099 1.0 0.9608   1.1 0.0217 *   
SNAP-25A AB003991 22,532 ± 3763 16,441 ± 1731 -1.4 0.0898   -1.4 0.0486 *  
  1. Key to abbreviations, * P < 0.05, ** P < 0.01, *** P < 0.001 (two-tailed unpaired t-test). Down regulated genes are expressed as negative fold changes.