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Table 1 Confirmation and comparison of ANOVA and paired t-test for microarray analysis.

From: Dorsal horn-enriched genes identified by DNA microarray, in situ hybridization and immunohistochemistry

Accession#

Description

D vs V Ratio

Confirmation

PANOVA

Ppair

Reference

K02248

Somatostatin

11.1

literature

<0.05

<0.05

[21–24]

S49491

Proenkephalin

4.9

literature

<0.05

<0.05

[25–28]

L09119

Neurogranin

4.1

literature

<0.05

<0.05

[29]

AF058795

GABA b2

2.8

literature

<0.05

<0.05

[30]

AB004267

CAMK I b2

2.7

literature

<0.05

<0.05

[31]

M15880

NPY

2.4

literature

<0.05

<0.05

[24, 32]

X04139

PKC

2.3

literature

<0.05

<0.05

[33][34]

M25890

Somatostatin

12.4

literature

<0.05

<0.05

[21–24]

X56306

Protachykinin

6.5

literature

<0.05

<0.05

[24, 27, 35]

M15191

Beta-tachykinin

5.0

literature

<0.05

<0.05

[24, 27, 35]

X55812

Cannabinoid receptor

2.9

literature

<0.05

<0.05

[36]

M16410

Neurokinin B

2.3

literature

<0.05

<0.05

[35, 37]

X62840

K channel Kv 3.1

2.2

literature

<0.05

<0.05

[38]

S79730

Nociceptin

2.1

literature

<0.05

<0.05

[39]

S39221

NMDA receptor NR1

2.5

literature

<0.05

<0.05

[40]

X57573

GAD

2.9

literature

<0.05

<0.05

[24, 41, 42][43]

AI102205

Vesicle associated calmodulin protein

4.6

Test confirmed

<0.05

<0.05

 

D17764

Synuclein beta

2.2

Test confirmed

<0.05

<0.05

 

AF023087

NGFI-A

2.2

Test confirmed

<0.05

<0.05

 

D12573

Hippocalcin

2.2

Test confirmed

<0.05

<0.05

 

AI639118

EST

2.5

Test confirmed

<0.05

<0.05

 

X54249

Zinc finger protein

2.4

Test not confirmed

<0.05

<0.05

 

X67241

Guanine nucleotide releasing factor

2.2

Not tested

<0.05

<0.05

 

L25633

Neuroendocrine-specific protein

2.0

Not tested

<0.05

<0.05

 

D10666

Neural visinin-like protein

2.4

Not tested

<0.05

<0.05

 

D90219

CNP

2.2

Not tested

<0.05

<0.05

 

AF007758

Synuclein-1

2.0

Not tested

<0.05

<0.05

 

U88958

Neuritin

2.0

Not tested

<0.05

<0.05

 

E13644

Neurodap-1

2.0

Not tested

<0.05

<0.05

 

AI639213

EST

3.1

Not tested

<0.05

<0.05

 

AF041107

Tulip2

2.1

Not tested

>=0.05

<0.05

 

AF019974

Chromogranin B

2.5

literature

<0.05

>=0.05

[44][45]

D10392

Syntaxin 1A

2.4

literature

<0.05

>=0.05

[46]

M93669

Secretogranin

2.4

literature

<0.05

>=0.05

[44, 47]

X62839

K-channel 3.1

2.3

literature

<0.05

>=0.05

[38]

AA894330

CAMKII beta

2.2

literature

<0.50

>=0.05

[31]

L09119

Neurogranin

2.1

literature

<0.05

>=0.05

[29]

AA925248

Sodium channel SCN6A

10.0

literature

<0.05

>=0.05

[48]

AF078779

4-repeat ion channel

6.9

Test confirmed

<0.05

>=0.05

 

S80376

G alpha(olf)

2.6

Test confirmed

<0.05

>=0.05

 

AI014091

MRG1

2.1

Not tested

<0.05

>=0.05

 

AI639036

EST

11.3

Not tested

<0.05

>=0.05

 

AI639062

EST

2.9

Not tested

<0.05

>=0.05

 

AI639470

EST

2.8

Not tested

<0.05

>=0.05

 

M20722

Proline rich protein

2.7

Not tested

<0.05

>=0.05

 

AF034899

Olfactory receptor (SCR D-9)

2.3

Not tested

<0.05

>=0.05

 

AF019043

Dynamin-like protein

2.2

Not tested

<0.05

>=0.05

 

AF091834

NSF

2.2

Not tested

<0.05

>=0.05

 

X67877

Resiniferatoxin-binding protein

2.1

Not tested

<0.05

>=0.05

 

AF089839

N-ethylmaleimide sensitive factor

2.1

Not tested

<0.50

>=0.05

 

AI072943

EST

2.1

Not tested

<0.05

>=0.05

 

AA866291

EST

2.0

Not tested

<0.05

>=0.05

 
  1. Genes that show greater than 2-fold expression in the dorsal spinal cord versus ventral spinal cord with P < 0.05 are listed for each analysis method. The gene expression fold increase for each probeset was calculated as the ratio of means of gene expression index between dorsal and ventral spinal cord.