Typical fluorescent micrographs of rhodamine labeled AChR distributions after stimulation with agrin or laminin-1 alone or in combination. See Table 2 for stimulus protocols. Note that the analysis is restricted to the perimeter of the images (Materials and Methods) A, no stimulus. There is a subtle presence of small aggregates about the cell perimeter (arrows). B, Agrin. C, laminin. D, Agrin and Laminin. E, Agrin followed by laminin. F, laminin followed by Agrin. All of these latter conditions consistently display larger, more intense receptor aggregates than the control cells in A.