Western blots analyses of NMDA receptor subunits in the mouse retina. (A),(B) and (C) Immunoblots of 6 μg of retinal SPM fraction proteins from controls and the rdta mice were first incubated with anti-NR1 antibody (A), and subsequently reprobed with anti-NR2A (B) and NR2B (C) antibodies, respectively. The immunolabeling densities were calculated as the integrated density values (IDV), and plotted in D. (D) The graph presents the changes in the antibody binding to NR1, NR2A and NR2B in littermate controls and the rdta mice. The average density calculated as IDV for the littermate controls was taken as 100. Values are means ± S.E.M (n = 3), P < 0.05, one way ANOVA with Bonferroni correction.