Western blot analysis of the immunolabeling of β-actin in the SPM fraction of the mouse retina. (A) Immunoblots of 6 μg of retinal SPM protein from control animals and the rdta mice were incubated with anti-β-actin antibody. The immunolabeling densities were calculated as the integrated density values (IDV) and plotted in B. (B) The graph presents the changes in the antibody binding to β-actin in three littermate control and three rdta mice. The average density calculated as IDV for the littermate controls was taken as 100%. The immunolabeling of β-actin in the SPM fraction is significantly increased in the rdta retina. Values are means ± S.E.M (n = 3), P < 0.05, one way ANOVA with Bonferroni correction.