Immuonolocalization of estrogen receptor and dopamine: Mixed neuronal cultures were immunostained as described in methods. (A) co-localization of dopamine (green) and estrogen receptor(ER) (red) in neurons. Dual staining cells appear yellow. (B) Immunostaining for dopamine. (C) immunostaining for ER. Scale bar in A = 30 μm and the scale bar in B and C = 50 μm.