Confocal microscope images of D-AKAP1 labeled NMJ in adult rat intercostal muscle. Anti-cytochrome c marks mitochondria and bungarotoxin labels the nAChR. (a) Distribution of D-AKAP1 at the NMJ and adjacent muscle. D-AKAP1 codistributes with cytochrome c in the actin region of the intercostal muscle and the postsynaptic region of the NMJ. (b) D-AKAP1 is enriched at the postsynaptic membrane, as shown by codistribution with nAChR. scale bar = 10 μm for both (a) and (b). (c) Double-labeling of phalloidin, which binds to F-actin, and D-AKAP1. Phalloidin demarcates the actin region and is used as a positive control for this region,(d) No primary control. Fluorophore-conjugated secondary antibodies show that very low background from nonspecific labeling comes from these antibodies. Bungarotoxin-TRITC was used to find the NMJs in the negative controls, scale bar = 10 μm for both (c) and (d).