Skip to main content

Advertisement

Figure 5 | BMC Neuroscience

Figure 5

From: Generation of choline for acetylcholine synthesis by phospholipase D isoforms

Figure 5

Overexpression of PLD1 in SN56 cells increases enzyme activity, acetylcholine synthesis and choline content in the medium. The cells were transfected with the hPLD1 expression plasmid as described in the Experimental Procedures. After 48 hours the cells were treated as described in Fig. 3 and PLD activity in intact cells (A), choline content of the medium (B), and intracellular ACh content (C), were measured. The data were analyzed by two-way ANOVA and Fisher's post hoc test and are expressed as average ± SEM. The asterisks indicate a significant difference between the PLD1 and mock transfectants (A-C). A. Effect of PLD 1 overexpression on PLD activity. The effect of PMA treatment is statistically significant in all groups. B. Effect of PLD1 overexpression on media choline level. The effect of PMA treatment is statistically significant in PLD1 transfectants. C. Effect of PLD1 overexpression on intracellular ACh level. The effect of PMA treatment is statistically significant in PLD1 transfectants.

Back to article page