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Figure 4 | BMC Neuroscience

Figure 4

From: Generation of choline for acetylcholine synthesis by phospholipase D isoforms

Figure 4

Overexpression of PLD2 in SN56 cells increases enzyme activity, acetylcholine synthesis and choline content in the medium. The cells were transfected with the expression plasmid as described in the Experimental Procedures. After 48 hours the cells were treated as described in Fig. 3 and PLD activity in intact cells (A), choline content of the medium (B), and intracellular ACh content (C), were measured. The data were analyzed by two-way ANOVA and Fisher's post hoc test and are expressed as average ± SEM. The asterisks indicate a significant difference between the PLD2 and mock transfectants (A-C). A. Effect of PLD2 overexpression on PLD activity. The effect of PMA treatment is statistically significant in all groups. B. Effect of PLD2 overexpression on media choline level. The effect of PMA treatment is statistically significant in PLD2 transfectants. C. Effect of PLD2 overexpression on intracellular ACh level. The effect of PMA treatment is statistically significant in PLD2 transfectants.

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