Developmental expression of synphilin-1 in different mouse strains. A. Northern blot of brain polyA+-RNA (500 ng per lane) from 129/Ola, ICR, and BL6JHUK mice at different developmental stages (P0, P8, P15, and adult). Probing with a 1.5 kB synphilin-1 probe detects a transcript of approximately 4 kB. RNA samples were pooled from 4 animals (at P0), or from 2 animals (at P8 and P15) from one litter. After stripping, the membrane was re-probed with an actin probe. B. Brain homogenate from 129/Ola, ICR, and BL6JHUK mice at P0, P8, P15, and adult age separated by 7.5% SDS-PAGE (30 μg protein per lane). The Western blot membrane was sequentially probed with polyclonal anti synphilin-1 and monoclonal anti α-tubulin antibodies. The recognized proteins have apparent molecular weights of approximately 85 kDa and 55 kDa, respectively.