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Table 1 Average concentration of Fe, Cu, Zn and Mn in large regions of the brain

From: Simultaneous monitoring of cerebral metal accumulation in an experimental model of Wilson’s disease by laser ablation inductively coupled plasma mass spectrometry

μg g−1wet weight Control WD %** change p- value
Entire section     
 Fe 14.1 ± 2.5 14.1 ± 1.0   .9
 Cu 4.2 ± 1.4 6.7 ± 1.5 +61% .0007
 Zn 13.2 ± 3.1 15.8 ± 1.7 +19% .07
 Mn 0.25 ± 0.07 0.26 ± 0.05   .4
Cortex (grey matter)
 Fe 12.4 ± 2.2 12.1 ± 0.8   .7
 Cu 2.7 ± 0.7 5.5 ± 1.2 +108% .00004
 Zn 13.9 ± 3.0 17.7 ± 0.8 +28% .008
 Mn 0.23 ± 0.06 0.26 ± 0.04   .2
Corpus callosum (white matter)*
 Fe 7.8 ± 1.1 7.6 ± 0.6   .6
 Cu 1.5 ± 0.5 2.9 ± 0.8 +102% .0004
 Zn 8.2 ± 2.1 9.5 ± 1.4   .2
 Mn 0.11 ± 0.03 0.13 ± 0.04   .2
Cerebellum, Cu rich regions lobules 4–5, 8-10
 Fe 17.4 ± 3.8 17.4 ± 2.1   1
 Cu 6.6 ± 1.6 10.9 ± 1.7 +64% .0001
 Zn 13.0 ± 2.9 16.0 ± 2.0 +23% .03
 Mn 0.28 ± 0.09 0.28 ± 0.04   .9
Cerebellum Cu poor regions (other)
 Fe 17.8 ± 3.5 16.3 ± 3.8   
 Cu 3.8 ± 0.9 7.5 ± 1.6 +95% .0002
 Zn 12.3 ± 2.7 13.8 ± 3.1   .3
 Mn 0.30 ± 0.08 0.27 ± 0.06   1
Cerebellar white matter*
 Fe 8.4 ± 1.9 6.9 ± 1.9   .1
 Cu 1.7 ± 0.3 4.0 ± 1.2 +132% .0004
 Zn 6.8 ± 1.4 7.9 ± 1.2   .1
 Mn 0.15 ± 0.04 0.13 ± 0.06   .9
Colliculus superior
 Fe 13.8 ± 2.2 13.3 ± 1.1   .7
 Cu 3.3 ± 0.7 6.1 ± 0.5 +85% .0005
 Zn 9.8 ± 2.0 11.4 ± 0.4   .08
 Mn 0.31 ± 0.07 0.34 ± 0.07   .3
Colliculus inferior
 Fe 15.7 ± 3.4 12.1 ± 1.5   .05
 Cu 3.6 ± 0.9 5.4 ± 0.7 +49% .003
 Zn 9.5 ± 2.1 11.2 ± 1.7   .1
 Mn 0.36 ± 0.10 0.35 ± 0.09   .06
Periventricular regions
 Lateral ventricules
  Cu 39 ± 17 17 ± 3 −56% .01
 Third ventricule
  Cu 50 ± 28 22 ± 4 −57% .02
 Fourth ventricule
  Cu 67 ± 22 19 ± 5 −71% .0004
  1. Concentrations of Fe, Cu, Zn, Mn (mean ± SD) measured by imaging LA-ICP-MS in regions of interest in the brain of 8 controls, and 9 WD mice, both on a SVJ129 background. Of each animal, one section was measured whereby each hemisphere was measured in an independent run with separate calibration standard. SD and heteroskedastic t-tests were calculated over the 8 and 9 individual bi-hemispheric averages.
  2. *white matter borders were delineated to minimize spillover from neighbour regions.
  3. **indicated, if > +10% or < −10% and p < .05.