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Table 1 Average concentration of Fe, Cu, Zn and Mn in large regions of the brain

From: Simultaneous monitoring of cerebral metal accumulation in an experimental model of Wilson’s disease by laser ablation inductively coupled plasma mass spectrometry

μg g−1wet weight

Control

WD

%** change

p- value

Entire section

    

 Fe

14.1 ± 2.5

14.1 ± 1.0

 

.9

 Cu

4.2 ± 1.4

6.7 ± 1.5

+61%

.0007

 Zn

13.2 ± 3.1

15.8 ± 1.7

+19%

.07

 Mn

0.25 ± 0.07

0.26 ± 0.05

 

.4

Cortex (grey matter)

 Fe

12.4 ± 2.2

12.1 ± 0.8

 

.7

 Cu

2.7 ± 0.7

5.5 ± 1.2

+108%

.00004

 Zn

13.9 ± 3.0

17.7 ± 0.8

+28%

.008

 Mn

0.23 ± 0.06

0.26 ± 0.04

 

.2

Corpus callosum (white matter)*

 Fe

7.8 ± 1.1

7.6 ± 0.6

 

.6

 Cu

1.5 ± 0.5

2.9 ± 0.8

+102%

.0004

 Zn

8.2 ± 2.1

9.5 ± 1.4

 

.2

 Mn

0.11 ± 0.03

0.13 ± 0.04

 

.2

Cerebellum, Cu rich regions lobules 4–5, 8-10

 Fe

17.4 ± 3.8

17.4 ± 2.1

 

1

 Cu

6.6 ± 1.6

10.9 ± 1.7

+64%

.0001

 Zn

13.0 ± 2.9

16.0 ± 2.0

+23%

.03

 Mn

0.28 ± 0.09

0.28 ± 0.04

 

.9

Cerebellum Cu poor regions (other)

 Fe

17.8 ± 3.5

16.3 ± 3.8

  

 Cu

3.8 ± 0.9

7.5 ± 1.6

+95%

.0002

 Zn

12.3 ± 2.7

13.8 ± 3.1

 

.3

 Mn

0.30 ± 0.08

0.27 ± 0.06

 

1

Cerebellar white matter*

 Fe

8.4 ± 1.9

6.9 ± 1.9

 

.1

 Cu

1.7 ± 0.3

4.0 ± 1.2

+132%

.0004

 Zn

6.8 ± 1.4

7.9 ± 1.2

 

.1

 Mn

0.15 ± 0.04

0.13 ± 0.06

 

.9

Colliculus superior

 Fe

13.8 ± 2.2

13.3 ± 1.1

 

.7

 Cu

3.3 ± 0.7

6.1 ± 0.5

+85%

.0005

 Zn

9.8 ± 2.0

11.4 ± 0.4

 

.08

 Mn

0.31 ± 0.07

0.34 ± 0.07

 

.3

Colliculus inferior

 Fe

15.7 ± 3.4

12.1 ± 1.5

 

.05

 Cu

3.6 ± 0.9

5.4 ± 0.7

+49%

.003

 Zn

9.5 ± 2.1

11.2 ± 1.7

 

.1

 Mn

0.36 ± 0.10

0.35 ± 0.09

 

.06

Periventricular regions

 Lateral ventricules

  Cu

39 ± 17

17 ± 3

−56%

.01

 Third ventricule

  Cu

50 ± 28

22 ± 4

−57%

.02

 Fourth ventricule

  Cu

67 ± 22

19 ± 5

−71%

.0004

  1. Concentrations of Fe, Cu, Zn, Mn (mean ± SD) measured by imaging LA-ICP-MS in regions of interest in the brain of 8 controls, and 9 WD mice, both on a SVJ129 background. Of each animal, one section was measured whereby each hemisphere was measured in an independent run with separate calibration standard. SD and heteroskedastic t-tests were calculated over the 8 and 9 individual bi-hemispheric averages.
  2. *white matter borders were delineated to minimize spillover from neighbour regions.
  3. **indicated, if > +10% or < −10% and p < .05.