Immunocytochemical staining and flow cytometer analysis of MSC-induced neural progenitor-like cells. (A) NPCs from group B (EGF + bFGF + IGF-1) strongly expressing nestin. (B) NPCs from group B also strongly express Sox-2. (C) Flow cytometer analysis of nestin expression of NPCs from all groups (A, B, C and D) indicating the early differentiation of MSCs into NPCs. Group E clearly show the existence of two populations of cells indicating partial differentiation due to the effect of neural basal media. Flow cytometric data further validated the results obtained from immunofluorescence staining for group B. Images were viewed at 100× magnification using a fluorescence microscope.