Figure 3

Immunoblot and immunocytochemical analysis of effect of CBL on Tau in Tau/GSK3β bigenic mice. (A) Representative Immunoblot image of total-tau (t-Tau), phospho-Tau (p-Tau) at epitopes recognized by the AT8 (Ser202/Thr205) and AT270 (Thr181) antibodies and actin levels in vehicle and CBL treated Tau/GSK3β bigenic mice in comparison to non-tg mice. (B- D) Image analysis of levels of t-Tau, p-Tau (AT8) and p-Tau (AT270) respectively in vehicle and CBL treated Tau/GSK3β bigenic mice in comparison to non-tg mice. (E) Immunohistochemical analysis of p-Tau (AT8) immunoreactivity in the hippocampus of vehicle and CBL treated Tau/GSK3β bigenic mice in comparison to non-tg mice. Boxes in upper panels are represented at higher magnification in the lower panels. (F) Image analysis of p-GSK3β immunoreactivity in the hippocampus of vehicle and CBL treated Tau/GSK3β bigenic mice in comparison to non-tg mice. (A, C) Scale bar in upper panels = 250 μm, in lower panels = 50 μm. (B, D) Error bars represent mean ± SEM (n = 6 per group, 6 m/o). *Indicates a significant difference (p < 0.05) between vehicle and CBL treated Tau/GSK3β bigenic mice analyzed by one-way ANOVA and Dunnett’s post hoc test.