Protein expression of mitochondrial biogenesis factors in XY and XX CGNs upon exposure to OGD (2 h) and reoxygenation (6–24 h). Western blot analyses of PGC-1α (A) and TFAM (B) were performed using total cellular extracts of XY and XX CGNs from either normoxia control or exposed to OGD/Reox for the indicated time points. Histograms show values for TFAM and PGC-1α normalized to actin. Values are expressed as mean ± SD from four independent experiments (*p < 0.05, ***p < 0.001 XX vs. XY, +p < 0.05, ++ p < 0.01 vs. control, a one-way ANOVA followed by Bonferroni/Dunn post hoc test was applied). Quantification of TFAM and PGC-1α specific bands showed increased expression in XY compared to XX CGNs.