Figure 4

Effects of OGD (2 h) followed by Reox (6–24 h) on transcription factors involved in mitochondrial biogenesis in XY and XX CGNs. A) PGC-1α, (B) TFAM and (C) NRF-1 mRNA expression was determined by RT-qPCR at indicated times as described in materials and methods. The PGC-1α, NRF-1 and TFAM mRNA expression (normalized to HPRT) were upregulated in XY as compared to XX CGNs during OGD/Reox. Results are mean ± SD (n = 4, *p < 0.05, **p < 0.01, ***p < 0.001, XX vs. XY, ⁺p < 0.05, ⁺⁺p < 0.01 vs. control, a one-way ANOVA followed by Bonferroni/Dunn post hoc test was applied).