HIV-1 Tat C mediated phosphorylation of adherens junction proteins (VE-cadherin and β-catenin). (A) Western blot analysis to check the effect of HIV-1 Tat C protein in hBMVECs, showing a dose dependent increase in tyrosine phosphorylation of VE-cadherin (p-Y-731). (B) The graph showing the result of densitometry for western blot images showing significantly induced tyrosine phosphorylation (p-Y-731) of VE-cadherin upon HIV-1 Tat/ heat inactivated Tat treatment. Quantitation of image densities have been done by ImageJ software normalized with β-tubulin. (C) Western blot analysis showing the insignificant effect of heat inactivated HIV-1 Tat C protein on tyrosine phosphorylation (p-Y-731) of VE-cadherin (D) Western blot analysis to show dose dependent increase in tyrosine phosphorylation of β-catenin (p-Y-654) in hBMVECs exposed to HIV-1 Tat C protein. (E) Densitometry analysis of image density to show change in tyrosine phosphorylation (p-Y-654) of β-catenin. Image densities have been normalized with β-tubulin. All the experiments have been performed in three independent biological sets and results are presented as mean ± S.E., *above bars are representing the p value ≤0.05 as level of significance, n = 3. (*for p value ≤0.05, **for p value ≤0.005).