Effects of mirtazapine on the striatal TH, DAT and VMAT2 protein expression examined by western blot analysis. (A), (B) and (C), representative membrane image (upper panels) and the densitometric analysis of the positive bands (lower graphs) of TH (A), DAT (B) and VMAT2 (C). In (A), (B) and (C), actin protein was used as an internal control. Expression of TH, DAT and VMAT2 proteins are expressed as % of vehicle (means ± SEM), n = 4–5 mice/group. Mirt.(4), mirtazapine 4 mg/kg; Mirt.(16), mirtazapine 16 mg/kg. Statistical significance was evaluated by one-way ANOVA followed by Scheffe test (F(TH) 4,17 = 16.115, F(DAT) 4,17 = 12.386, F(VMAT2) 4,19 = 6.711 **P < 0.01 compared with MPTP-treated group) or by Student’s t-test (#P < 0.05 compared with MPTP-treated group).