Figure 6

Treatment with HDAC inhibitors induces reporter expression in 1A4s2 and 3E2s rBDNF-hRluc-EGFP cell lines. (A, B, C, D) Fold luciferase activity in HDAC inhibitor treated cells relative to vehicle treated cells (dashed line) measured in live cells during 12 hours. 1A4s2 and 3E2s cells were treated with HDAC inhibitors apicidin (100 nM) (A), SAHA (1 μM) (B), TSA (100 nM) (C), sodium valproate (1 mM) (D) and vehicle control (0.1% DMSO or water) together with Enduren live cell substrate. Error bars indicate SD of three independent experiments (* p < 0.01, # p < 0.05, relative to vehicle treated control). (E, F) Cell viability in HDAC inhibitor treated cells relative to vehicle treated cells after 12 hours of treatment in 1A4s2 cell line (E) and 3E2s cell line (F). Viability was assayed by cellular ATP levels. Error bars indicate SD of three independent experiments.