Analysis of transgene copy number and chromosomal state. (A) qPCR analysis of transgene copy number in different rBDNF-hRluc-EGFP cell lines. Error bars show SE of three technical replicates. (B) FISH analysis of reporter construct chromosomal status in different rBDNF-hRluc-EGFP cell lines. Hybridisation was performed with rBDNF-hRluc-EGFP BAC specific probe (red signal) and DNA was stained with Hoecht 33342 (blue signal).