Figure 1From: BAC-based cellular model for screening regulators of BDNF gene transcriptionSchematic drawing of the rBDNF-hRluc-EGFP-BAC construct used for generation of HeLa cell-lines expressing the transgene. (A) The BAC used contained the rat BDNF locus spanning 13Â kb upstream of the first exon and 144Â kb downstream of the last polyadenylation signal. The exonic structure of rat BDNF gene is adapted from [11]. (B) The coding region of BDNF was replaced by homologous recombination with sequence coding for humanised Renilla luciferase, Ala-Ala-Ala-Thr linker and EGFP fusion protein. Protein coding regions are shown as gray (BDNF), orange (hRluc) or green (EGFP) boxes. Untranslated regions are shown as white boxes.Back to article page