Immunofluorescence localization of tau and model Hirano bodies. H4 cells were transiently transfected with FLAG-tagged tau constructs and CT-GFP to induce model Hirano bodies. 24 h post-transfection, cells were fixed and stained for good preservation of F-actin. Tau was stained with anti-FLAG primary antibody and Alexa Fluor350 secondary antibody (blue). F-actin was stained using TRITC-phalloidin (red). All tau isoforms colocalize with model Hirano bodies except 352PHP, which is localized diffusely throughout the cell. Tau variants examined were control tau 352WT, 441WT, or tau mutants K18, R5H, G272V, P301L, R406W, K18ΔK280, and 352PHP. Scale bar = 20 μm.