Model Hirano bodies differentially influence cell death in the presence of GSK3β (S9A) and tau. H4 cells were transiently transfected with equal amounts of plasmid DNA. Control cells (white bars) were transfected with plasmid encoding either GFP or model Hirano bodies (CT-GFP) in the presence or absence of constitutively active GSK3β (S9A). Cells were transfected with plasmid encoding GSK3β (S9A), a single tau variant, and either GFP (black bars) or model Hirano bodies (check bars). A. Tau variants 352WT, 441WT, or 352PHP. B. Tau variants R5H, G272V, P301l, or R406W. C. Tau variants K18 or K18ΔK280. D. Tau variants 441WT or P301L transfected with a lower amount of plasmid encoding GSK3β (S9A). Model Hirano bodies significantly promote cell death induced by GSK3β (S9A) and tau except in tau mutants R5H, R406W, K18, and K18ΔK280, where they have no effect. *p < 0.05, **p < 0.01, ***p < 0.001. Error bars represent the standard deviation.