Transverse sections through the spinal cord with adjacent transplant. bNCSCs (green) located outside the spinal cord do not express the Schwann cell marker p75 (a; red p75, eGFP bNCSCs, blue Hoechst), are arranged in elongated tubes (arrowheads) and express glial markers GFAP (b, red), and Sox2 (c, red), or as clusters or single cells and express TrkB (d, red). Inserts a-c are merged images of eGFP-positive cells, the respective antibody labeling and Hoechst labeling. Insert in d shows higher magnification of TrkB expressing bNCSCs. Scale bar in a-d = 50 μm; inserts = confocal images showing close association of bNCSC with Schwann cells (a), GFAP-expressing bNCSCs in the tubes (b), Sox2-expressing bNCSCs in tubes (c), TrkB-expressing bNCSCs in the PNS compartment (d).