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Figure 5 | BMC Neuroscience

Figure 5

From: Oxidative and pro-inflammatory impact of regular and denicotinized cigarettes on blood brain barrier endothelial cells: is smoking reduced or nicotine-free products really safe?

Figure 5

Effect of CSE exposure (24 h) on endothelial expression and distribution of VE-cadherin and Claudin-5. (A) Immunofluorescence analysis of BBB endothelial cultures revealed a significant up-regulation of VE-cadherin (at cell-cell junctions) and claudin-5 expression following exposure to 3R4F, NF and ultralow nicotine CSEs. (B) Immunofluorescence analyses were confirmed by WB of corresponding membrane fractions. n = 3 biological replicates, *p < 0.05, **p < 0.01 compared to controls.

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