Calpain expression after soman and antidotes treatment. Efficacy of HI-6, atropine and midazolam on soman induced calpain protein levels of rats sacrificed at 30 min, 2.5 h, 1d and 7d time points (n = 4 per each time point). Calpain immunoreactivity levels in the rat hippocampus (A), cerebral cortex (B) and cerebellum (C) after soman poisoning (E- bar graph). Calpain levels in the rat hippocampus (F), cerebral cortex (G) and cerebellum (H) after antidotes treatment (J-bar graph). β-actin (D and I), was used as protein loading control. A difference of p < 0.05 was considered significant (*).