Efficacy of antidotes on soman induced c-Fos and Bax mRNA levels. Effect of HI-6 (50 mg/kg, i.m), atropine (10 mg/kg, i.m) and midazolam (5 mg/kg, i.m) on soman induced Bax (Figure 4A) c-Fos (Figure 4B) mRNA levels of rats sacrificed at 30 min, 2.5 h, 1d and 7d time points (n = 4 per each time point). Quantitative real-time RT-PCR was carried out and results were presented as fold change over control (presented as 1.0 fold) after normalizing the data (n = 4 per each time point) with housekeeping gene, RNA polymerase-II (RP-II), which did not show any significant changes in the mRNA expression among control and experimental groups (Data not presented). *Significantly different from control group. A difference of p < 0.05 was considered significant.