Figure 2
From: Nanoscopic spine localization of Norbin, an mGluR5 accessory protein
Norbin localizes to and accumulates in dendritic spines of hippocampal neurons. A) Confocal images of neurons showing immunolabeling of Norbin and PSD-95. Norbin is localized in the soma and dendrites. B) A higher magnification shows that Norbin is localized to dendritic spines. C-D) Confocal images of neurons co-transfected with Norbin-GFP and TagRFP showing Norbin accumulated in spines (arrows). E) An intensity plot of the line in D shows the relative accumulation of Norbin-GFP in spines. F-I) Fluorescent recovery after photobleaching (FRAP) experiments in neurons co-transfected with Norbin-GFP and mCherry. F) In dendrites, there was no difference in recovery half times between Norbin and mCherry (tNorbin = 3.7 ± 0.2 s, tmCherry = 3.2 ± 0.2 s, mean ± SEM, p > 0.5, Kruskal-Wallis test, n = 11). G, H) In spines, recovery half times were significantly different between Norbin and mCherry (tNorbin = 8.0 ± 0.4 s and tmCherry = 1.9 ± 0.2 s, mean ± SEM, ***p < 0.0001, Kruskal-Wallis test, n = 59). I) Norbin-GFP had a longer half time of recovery in spines than in dendrites (8.0 ± 0.4 s vs. 3.7 ± 0.2 s, mean ± SEM, *p < 0.05, Kruskal-Wallis test).