Figure 5
Quantitation of viral transduction depicted in Figure4. In this experiment, AAV-RFP virus was pseudotyped as AAV2/1, 2/2, 2/5, 2/7, 2/8, 2/9, 2/rh10, 2/DJ, or 2/DJ8 and infused into the BLA at the highest titer achieved for each respective serotype (see Figure 2C). (A). Viral transduction (OD * area (μm2)) was quantified for each viral infusion and these data were averaged across infusions and reported as mean viral transduction. These measurements included measuring the entire amount of transduction (Total) and the viral transduction that was confined to the BLA (BLA only). These data are displayed side-by-side for each serotype. (B). Viral OD was quantified for each viral infusion and these data were averaged across infusions and reported as mean viral OD. These measurements included measuring the OD for the entire amount of transduction (Total) and the OD for the viral transduction that was confined to the BLA (BLA only). These data are displayed side-by-side for each serotype. (C). Viral spread (area (μm2)), was quantified for each viral infusion and these data were averaged across infusions and reported as mean viral spread. These measurements included measuring the viral spread for the entire viral transduction (Total) and the viral spread for the viral transduction that was confined to the BLA (BLA only). These data are displayed side-by-side for each serotype. (D). For analysis of viral transduction across the anterior-posterior axis of the BLA, the amygdala was subdivided into 5 regions with respect to Bregma: -2.3 (-1.8 - -2.7); -2.8 (-2.7 - -3.1); -3.3 (-3.1 - -3.4); -3.6 (-3.4 - -3.7); 3.8 (-3.7 - -4.0) (see Figure 3) and the total BLA only transduction was quantified within each subregion. Graphs displayed in A. C. and D. have logarithmic scales. Error bars represent the standard error of the mean.