Figure 2From: Adeno-associated viral serotypes produce differing titers and differentially transduce neurons within the rat basal and lateral amygdalaAAV serotypes produce differing titers of virus following laboratory production. (A). Schematic diagram of AAV2 genome plasmids used in this study. AAV-RFP contains a red fluorescent protein gene (dsRed-Express) controlled by a mouse αCaMKII promoter that has been previously described [41]. AAV-misc has been previously described [43]. Both AAV2 genome plasmids contain genomes that span ~3300 bps (left inverted terminal repeat (ITR) to right ITR). (B). Viral titers obtained following iodixanol purification. (C). Viral titers obtained in (B). displayed as a table. (D). Viral titers obtained from crude viral lysates following small scale viral production. AAV2/2 produces significantly less packaged virus compared to the other serotypes examined. ANOVA p < .0001; Fisher’s Post-hoc test p < .05 for AAV2/2 and AAV2/2* compared to all other groups. n = 3. Error bars equal standard error of the mean (SEM). (E) AAV2/1 viral titers are ~ 10 fold less compared to AAV2/DJ8 viral titers following full scale production and iodixanol gradient purification. Viral titers for AAV-RFP viruses pseudotyped as AAV2/1 and AAV2/DJ8 (n = 1) are displayed next to viral titers for AAV-misc viruses pseudotyped at AAV2/1 (n = 2) and AAV2/DJ8 (n = 3). Error bars = SEM. Graphs displayed in B. D. and E. have logarithmic scales.Back to article page