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Table 1 Steady-state voltage-dependent activation parameters of the rEag1 and rEag2 chimeric channels

From: The punctate localization of rat Eag1 K+channels is conferred by the proximal post-CNBHD region

  V0.5(mV) k(mV) n
rEag1-WT -17.5 ± 0.9 19.9 ± 1.5 16
rEag1-I -19.2 ± 0.8 17.8 ± 1.2 17
rEag1-II -8.5 ± 0.7* 24.6 ± 0.7* 11
rEag1-III -16.1 ± 0.6 14.3 ± 1.5* 15
rEag1-IV -15.8 ± 1.3 18.3 ± 1.0 12
rEag2-WT -43.6 ± 1.2 18.3 ± 1.1 18
rEag2-I -40.0 ± 0.7 17.8 ± 0.9 10
rEag2-II -42.6 ± 0.5 19.0 ± 0.7 14
rEag2-III -54.1 ± 1.8* 19.1 ± 1.4 15
rEag2-IV -44.8 ± 1.4 18.5 ± 1.3 14
  1. To study steady-state voltage-dependent activation of channels, isochronal tail currents were normalized to the maximum amplitude to obtain the fraction of open channels (Po) at the indicated membrane potential. Data points were fit with a Boltzmann equation: Po(V) = 1/{1 + exp[(V0.5-V)/k]}, where V0.5 is the half-maximal voltage for activation, and k the slope factor of the Po-V curve. Data are shown as mean ± SEM (*, significantly different from the corresponding wild-type (WT); t-test: p < 0.05).