Intracellular calcium responses to BzATP in adult human astrocytes. (A) Representative change in intracellular Ca2+ ([Ca2+]i) (response collated from 21 cells) to BzATP (applied at 300 μM). The amplitude of response, expressed as a ratio of fluorescent intensity 340/380, was 0.23. (B) Representative change in [Ca2+]i (response collated from 25 cells) to BzATP in the presence of lipopolysaccharide (LPS at 100 ng/ml, pretreatment for 16 h). The amplitude of response (F340/380) was 0.26 from baseline to plateau level. Data shown in this figure was recorded from cells obtained and cultured from one surgical case.