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Figure 1 | BMC Neuroscience

Figure 1

From: Microparticles in multiple sclerosis and clinically isolated syndrome: effect on endothelial barrier function

Figure 1

Detection of PMPs and EMPs in plasma. (A-C) Flow cytometry scattergraphs for the quantitation of PMPs and EMPs-CD31. MPs smaller than 3 μm were identified by cytometry in the presence of 3 μm diameter beads (left graphs, squared area). These MPs were positive for Annexin V (central graphs, squared areas). Additional incubation with anti-CD31 and CD42 antibodies (A, B, right graphs) yielded two populations: AnxV+ MPs positive for CD31 and CD42 (Q2), which suggests a platelet origin for this MP subpopulation (PMP), and AnxV+ MPs, positive for CD31 and negative for CD42 (Q4), which suggests an endothelial origin (EMP). Prior incubation with an antibody isotype control (iso) yielded no positive staining (C). (A) Plasma from healthy control, (B, C) plasma from multiple sclerosis patient (RR) (D-F) Flow cytometry scattergraphs showing the identification of EMPs-CD62. A subset of MPs smaller than 3 μm beads (top graphs, squared area) was positive for an anti-CD62E antibody (CD62E) (D, E, bottom graphs, squared areas) and negative for an antibody isotype control (iso) (F, bottom graph, squared area). (D) Plasma from a healthy control (E, F) plasma from multiple sclerosis patient (RR).

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