Figure 8
From: Neurotrophins regulate ApoER2 proteolysis through activation of the Trk signaling pathway
Reelin induces ApoER2 proteolysis in PC12 cells without affecting p75 CTF levels. Western blot of cell lysates from PC12 cells stably expressing HA-ApoER2. Serum-starved cells were pre-treated with 10 μM DAPT and then incubated with recombinant reelin or the control medium (mock) for 2 h. ApoER2 (A) and p75 (D) were recognized using an antibody directed against its intracellular region. α-tubulin is shown as a loading control. (B and C) Quantification of blot levels of ApoER2 and its CTF; both quantifications were normalized to the loading control α-tubulin and plotted as the average ± SD of three independent experiments. Student’s t-test, **P < 0.005, ***P < 0.001. (E and F) Quantification of the blot levels of p75 and its CTF; both quantifications were normalized to the loading control α-tubulin and plotted as the average ± SD of three independent experiments. Student’s t-test, not significant (N.S).