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Figure 5 | BMC Neuroscience

Figure 5

From: Hypocretin1/orexinA-immunoreactive axons form few synaptic contacts on rat ventral tegmental area neurons that project to the medial prefrontal cortex

Figure 5

Subcellular location of Hcrt1/OxA-immunoreactivity in axonal profiles of the ventral tegmental area. (A) Hcrt1/OxA location in an axon sectioned so that the same image contains a preterminal varicose enlargement (Hcrt1/OxA-V; 1; diameter = 0.347 μm) connected to a short narrowing of the axon (2; diameter = 0.125 μm). (B) Hcrt1/OxA-immunolabeled transverse-sectioned unmyelinated axons (Hcrt1/OxA-Ax). Note that one of the axons only shows the Hcrt1/OxA reaction product in some parts of its cytoplasm (Hcrt1/OxA-Ax1), while the other (Hcrt1/OxA-Ax2) is completely filled by DAB-nickel-immunoprecipitate. (C) Longitudinally-sectioned Hcrt1/OxA unmyelinated axon. (D) Hcrt1/OxA-immunolabelled axon terminal (Hcrt1/OxA-T) that makes an asymmetric synapse (curved arrow) on an unlabelled dendrite (Den). Note the presence of Hcrt1/OxA-immunoreaction product in dense-cored vesicles (dcv), large dcv (ldcv) and in specific regions of the cytoplasm. (E) Hcrt1/OxA-immunoreactivity is observed exclusively within large dense-cored vesicles (ldcv) of an axon terminal (Hcrt1/OxA-T). (F) Varicosity showing a dense DAB-nickel precipitate for Hcrt1/OxA in ldcv and the cytoplasm. Scale bar, A-C, E-F,0.2 μm; D, 0.5 μm.

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BMC Neuroscience

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