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Figure 4 | BMC Neuroscience

Figure 4

From: Hypocretin1/orexinA-immunoreactive axons form few synaptic contacts on rat ventral tegmental area neurons that project to the medial prefrontal cortex

Figure 4

Hcrt1/OxA immunoreactivity in the ventral tegmental area (VTA). (A) Coronal section showing Hcrt1/OxA-immunoreactive fibers in the VTA. (B) High magnification of boxed area in A illustrating Hcrt1/OxA-containing axons. Arrowheads point to Hcrt1/OxA varicose axons in the parabrachial subdivision of VTA. (C) Double immunolabeling for Fluorogold (DAB, brown immunoprecipitate) and Hcrt1/OxA (DAB-nickel, black immunoprecipitate) in the VTA. (D) High magnification of boxed area in C, showing an Hcrt1/OxA containing axon (arrowhead) close to a Fluorogold-labeled neuron (arrow) within the parabrachial subdivision of VTA ipsilateral to the injection site. IP, interpeduncular nucleus; PAG, periaqueductal gray; PN, paranigral subdivision of the VTA; CLi, caudal linear raphe nucleus; cp, cerebral peduncle; RN, red nucleus. Scale bar, A,C, 500 μm; B,D, 100 μm.

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BMC Neuroscience

ISSN: 1471-2202

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