B355252 restores glutamate-evoked reduction of ERK3 expression in HT-22 cells. Cultured cells were treated with glutamate, with and without B355252. Cell lysates were prepared, subjected to SDS-PAGE, and immunoblotted with ERK3 specific antibody. Membrane was reprobed with anti-GAPDH and used to normalize gel loading. The bar graph shows the relative levels of Erk3 in the samples. Glutamate-dependent attenuation of ERK3 was reversed by treatment with B355252 (8 μM). B355252 exclusively had no significant effect on the basal level of ERK3 (***p<0.001 compared to glutamate-treated cells).