Figure 5

Glutamate-evoked production of ROS is blocked by B355252 in HT-22 cells. HT-22 was exposed to B355252 for 2 h, and then treated with glutamate for 10 h. ROS level was measured with CellRox Deep Red. Glutamate-dependent elevation of ROS was significantly attenuated by pretreatment of cells with B355252 (8 μM). B355252 alone did not significantly affect ROS level in the cell (***p<0.001 compared to glutamate-treatment; ###p<0.001 compared to control).