Figure 4

B355252 inhibits glutamate-induced accumulation of intracellular Ca²⁺. HT-22 was exposed to glutamate alone and in the presence of B355252. Changes in intracellular Ca²⁺ load was measured with the calcium binding dye Fura-2. Accumulation in Ca²⁺ by glutamate treatment was significantly attenuated by 8 μM of B355252. B355252 alone did not alter Ca²⁺ homeostasis in the cell. (***p<0.001 compared to glutamate-treated cells; ###p<0.001 compared to control).